Hypermethylation involved in DNA profiles of lung cancer specific tumour suppressor genes and epigenetic modification caused by an ultra-highly diluted homeopathic drug, Condurango 30C, in vitro and in vivo

Background and objectives: DNA hyper-methylation is an important aspect involved in carcinogenesis cancer progression, which affects mainly CpG islands of causes inactivation tumour suppressor genes. Therefore hypermethylation status the genomic both transformed cancerous cell lines carcinogen-induced lung was ascertained by analysis expressions certain major specific The other objective to examine if ultra highly diluted homeopathic drug, Condurango 30C, had ability modulate methylation. Methods: methylation activity, any, has been H460-NSCLC cells vitro BaP-induced rats vivo, respect genes like p15, p16, p18 p53 using PCR-SSCP analyses. modulation methylation, 30C also verified against placebo control a blinded manner. Results: 30C-treated showed significant decrease band-intensity p15 especially methylated condition, vitro, at IC50 dose (2.43µl/100µl). SSCP supported state, vitro. Inhibition observed after post treatment rat with 30C. results gave better indication differences band-position single strand separation treated samples. Conclusion: could trigger epigenetic modification via but placebos not.